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美國(guó)哈佛儀器旗下BTX公司自1983年成立以來(lái),以電穿孔儀、電融合儀為主要產(chǎn)品,是第壹家生產(chǎn)高質(zhì)量電穿孔儀器的生產(chǎn)廠家,開(kāi)創(chuàng)了許多在電穿孔、電融合、轉(zhuǎn)染、轉(zhuǎn)化等方面的新產(chǎn)品和技術(shù),不僅為全球在此領(lǐng)域的研究者提供了BTX專業(yè)產(chǎn)品,而 且擁有多至25種的配件,包括:電穿孔、電融合、活體導(dǎo)入配件等等,成為全球唯壹專業(yè)的轉(zhuǎn)基因、電融合、活體轉(zhuǎn)基因儀及配件的生產(chǎn)廠家。
詳情介紹:
Agile Pulse In Vivo系統(tǒng)是疫苗和免役接種研究者的理想選擇。系統(tǒng)根據(jù)皮下接種(ID)和肌肉接種(IM)的應(yīng)用特點(diǎn)分別配置了特別設(shè)計(jì)的針形矩陣電極,并且對(duì)電轉(zhuǎn)染儀的程序進(jìn)行了特別優(yōu)化,帶給您更高的轉(zhuǎn)染效率。電穿孔技術(shù)配合質(zhì)粒注射技術(shù)使用,在基因表達(dá)及持續(xù)性和產(chǎn)生免役原性上顯示出高達(dá)100倍的增長(zhǎng)。Pulse Agile技術(shù),首先利用短時(shí)間高強(qiáng)度的脈沖使細(xì)胞膜產(chǎn)生通透性,再利用低強(qiáng)度脈沖促進(jìn)DNA導(dǎo)入細(xì)胞,配合多針矩陣電極的使用,大大促進(jìn)了抗原在細(xì)胞內(nèi)的表達(dá)。Agile Pulse系統(tǒng)配有豐富多樣的多針矩陣電極選擇,并且內(nèi)置電阻測(cè)量技術(shù),大大促進(jìn)了皮下和肌肉免役接種技術(shù)。

Agile Pulse In Vivo系統(tǒng)能有效增強(qiáng)免役反應(yīng)并縮短免役接種程序,從而極大提高您的實(shí)驗(yàn)效率。

 

Agile Pulse In Vivo系統(tǒng)廣泛應(yīng)用于:

皮下DNA疫苗接種

肌肉DNA疫苗接種

肌肉DNA疫苗接種

Drug導(dǎo)入

 

特點(diǎn):

方波

多重方波

電阻/電擊脈沖監(jiān)控

實(shí)驗(yàn)日志存儲(chǔ)

無(wú)限量自定義程序存貯

遠(yuǎn)程控制

 

電穿孔應(yīng)用:

真核細(xì)胞

活體(活體電極)

皮下接種(針形矩陣電極)

肌肉接種(針形矩陣電極)

 

技術(shù)參數(shù):

用戶界面:觸摸屏

電壓:50 - 1000 v

脈沖時(shí)間:5 μs - 10 ms

脈沖間隔時(shí)間:20 μs - 1 s

數(shù)據(jù)輸出:USB

產(chǎn)品尺寸(長(zhǎng)x x高):12.6 x 7.9 x 15.7 in

重量:25 lbs

工作溫度:10° - 40° C

輸入電壓:100 - 250 VAC

 


已發(fā)表文獻(xiàn):

Bongard N, Lapuente D, Windmann S, Dittmer U, Tenbusch M, Bayer, W. Interference of Retroviral Envelope with Vaccine-Induced CD8+ T Cell Responses is Relieved by Co-Administration of Cytokine-Encoding VectorsRetrovirology. 2017;14: 28.

 

Eyquem J, et al. Targeting a CAR to the TRAC Locus with CRISPR/Cas9 Enhances Tumour Rejection. Nature. 2017;543: 113-117.

 

Juillerat A, et al. An Oxygen Sensitive Self-Decision Making Engineered CAR T-CellSci Rep. 2017 Jan 20;7: 39833.

 

Denies S, et al. Evaluation of a Xenogeneic Vascular Endothelial Growth Factor-2 vaccine in Two Preclinical Metastatic Tumor Models in MiceCancer Immunol Immunother. 2017 Dec;66(12): 1545-1555.

 

Juillerat A, et al. Design of Chimeric Antigen Receptors with Integrated Controllable Transient FunctionsSci Rep. 2016 Jan 11;6: 18950.

 

Peterson CW, et al. Long-Term Multi-Lineage Engraftment of Genome-Edited Hematopoietic Stem Cells after Autologous Transplantation in Nonhuman PrimatesBlood. 2016 May 19;127(20): 2416-2426.

 

Hawksworth DJ, Ziemann R, Manoj S. Enhanced Immune Response Following DNA Immunization Through In Vivo ElectroporationMonoclon Antib IImmunodiagn Immunother. 2016 Jul 27;35(5): 239-244.

 

Juillerat A, et al. Design of chimeric antigen receptors with integrated controllable transient functionsSci Rep. 2016 Jan 11;6: 18950.

 

Christopher W, et al, Long-term multilineage engraftment of autologous genome-edited hematopoietic stem cells in nonhuman primatesBlood. 2016;127: 2416-2426.

 

Wang L, et al. Evaluation of Candidate Vaccine Approaches for MERS-CoVNat Comm. 2015 Jul28;6:7 712.

 

Davtyan H, et al. BTX AgilePulse(TM) System is an Effective Electroporation Device for Intramuscular and Intradermal Delivery of DNA Vaccine. Curr Gene Ther. 2014;14(3): 190-199.

 

Daftarian MP, Vosoughi A, Lemmon V. Gene-Based Vaccination and Screening Methods to Develop Monoclonal Antibodies. Methods Mol Biol. 2014;1121: 337-346.

 

Johansson DX, Ljungberg K, Kakoulidou M, Liljestrom P. Intradermal Electroporation of Naked Replicon RNA Elicits Strong Immune ResponsesPLoS One, 2012 Jan 4;7(1): e29732.

 

Lladser A, Ljungberg K, Tufvesson H, Tazzari M, Roos A, Quest FG, Kiessling R. Intradermal electroporation with a survivin DNA vaccine induces CTLs against a self-epitope, suppresses angiogenesis and confers long-term protection against mouse melanomaCancer Immunol Immunother. 2010 Jan;59(1): 81-92.

 

Lundberg K, Roos A-K, Pavlenko M, Wehrum D, Pisa P. A modified epitope identified for generation and monitoring of PSA-specific T cells in patients on early phases of PSA-based immunotherapeutic protocolsVaccine. 2009 Mar 4;27(10): 1557-65.

 

Br?ve A, Halleng?rd D, Gudmundsdotter L, Stout R, Walters R, Wahren B, Hallermalm K. Late administration of plasmid DNA by intradermal electroporation efficiently boosts DNA-primed T and B cell responses to carcinoembryonic antigenVaccine. 2009 Jun 8;27(28): 3692-6.

 

Roos, A-K, Eriksson, F, Walters, D, Pisa, P, King, A. Optimization of skin electroporation in mice to increase tolerability of DNA vaccine delivery to patientsMolecular Therapy. 2009 Sep;17(9): 1637-42.




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